Key Responsibilities:
* Isolate Genomic DNA from various sources including blood and established cell lines.
* Conduct commonly used molecular biology techniques such as bacterial growth, plasmid DNA preparation, restriction enzyme digestion, PCR, gel electrophoresis, etc.
* Perform gene expression using both transient and stable mammalian systems.
* Conduct protein purification and characterization with basic biochemical and biophysical techniques such as SDS-PAGE, Western Blot, ELISA, spectrophotometry, etc.
* Characterize and manage antibody and DNA inventories.
* Maintain mammalian cell culture including thawing, expansion, characterization, harvesting, freezing, and inventory management.
* Characterize membrane antigen expression using Flow Cytometry.
* Prepare media, buffers, and reagents.
* Use One Lambda products and research tools for HLA Molecular and Serological Typing.
* Automate routine protocols to increase capacity and improve consistency.
* Compile and analyze data to complete update or report.
* Perform all activities in compliance with GDP/GMP, regulatory and safety policies.
Basic Requirements:
* B.S. degree in molecular biology, cell biology, biochemistry, genetics, immunology or a related discipline in Biological Sciences and Engineering
* 1-2 years of laboratory experience preferred
* Proficiency in Microsoft Office
* Excellent organizational and record keeping skills
* Capable of multi-tasking and good time management
* Flexible with changing plans and schedules
* Good oral and written communication skills
Waltham, MA
Thermo Fisher Scientific Inc. (NYSE: TMO) is the world leader in serving science, with revenues of more than $24 billion and approximately 70,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, deliver medicines to market and increase laboratory productivity. Through our premier brands - Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services - we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive services.
To serve science, Thermo Fisher Scientific needs to stay ahead of it. To be the world leader in serving science, we need to anticipate (pre-empt) customer needs. We need to constantly think about advancing science, so customers have the freedom to be bolder and more innovative – we are committed to pushing science and technology a step beyond where it is today.