Senior Associate Scientist - Protein Expressionsion
Posted on: 18 Feb 2021
Cambridge, MA
Job Description
At Bristol Myers Squibb, we are inspired by a single vision transforming patients lives through science. In oncology, hematology, immunology and cardiovascular disease and one of the most diverse and promising pipelines in the industry each of our passionate colleagues contribute to innovations that drive meaningful change. We bring a human touch to every treatment we pioneer. Join us and make a difference.
Position Responsibilities:
* Mammalian protein expression in shake flasks using both transient transfection and stable cell lines (Expi293, ExpiCHO and CHO-S)
* Generation of stable pools for protein expression
* Operation and monitoring of stir-tank as well as wave-bag bioreactors (3L-50L). Sampling for analysis of nutrients and dissolved gasses in culture media
* Harvest of cell culture using filtration and centrifugation
* Execute automated methods for transient protein expression. Assist in method development experiments for OpenTron, Janus and Biomek Liquid Handlers
* Routine maintenance and cell banking of HEK and CHO cells
* Ensure accurate registration of protein lots and associated data into LIMS.
* Maintain a complete and current Electronic Laboratory Notebook.
Qualifications:
* Candidate should have a B.S. in Biology, Engineering or a related field with 1 to 5+ years of relevant experience in an industry setting
* Experience with Mammalian cell culture is required
* Prior experience with stable cell line/pool development, bioreactor operation and harvesting procedures such as TFF and/or depth filtration would be an advantage
* Desire to operate automation systems and develop methods would also provide an advantage
* Experience with LIMS and ELN is highly desirable
* Excellent time management skills and a demonstrated ability to work in a team environment
* Excellent interpersonal and communication skills along with a sense of urgency and ability to work under tight deadlines
* Must be detail oriented
* Basic protein characterization experience, such as, SDS-PAGE _ Preferred
Around the world, we are passionate about making an impact on the lives of patients with serious diseases. Empowered to apply our individual talents and diverse perspectives in an inclusive culture, our shared values of passion, innovation, urgency, accountability, inclusion and integrity bring out the highest potential of each of our colleagues.
Bristol Myers Squibb recognizes the importance of balance and flexibility in our work environment. We offer a wide variety of competitive benefits, services and programs that provide our employees with the resources to pursue their goals, both at work and in their personal lives.
Bristol-Myers Squibb
New York, New York
Bristol-Myers Squibb Company discovers, develops, licenses, manufactures, markets, distributes, and sells biopharmaceutical products worldwide. The company offers drugs in oncology, immunoscience, cardiovascular, and fibrotic diseases. The company’s products include Opdivo, a biological product for anti-cancer indications; Eliquis, an oral inhibitor targeted at stroke prevention in adult patients with non-valvular atrial fibrillation, and the prevention and treatment of venous thromboembolic disorders; and Orencia, a biological product for adult patients with moderately to severely active RA and prostate-specific antigen, as well as reducing signs and symptoms in certain pediatric patients with moderately to severely active polyarticular juvenile idiopathic arthritis.
It also provides Sprycel, a tyrosine kinase inhibitor for the treatment of Philadelphia chromosome-positive chronic myeloid leukemia; Yervoy, a monoclonal antibody for the treatment of patients with unresectable or metastatic melanoma; Empliciti, a humanized monoclonal antibody for the treatment of multiple myeloma; and Baraclude, an oral antiviral agent for the treatment of chronic hepatitis B. In addition, the company offers Reyataz, a protease inhibitor for the treatment of human immunodeficiency virus (HIV) and Evotaz; Sustiva franchise, a non-nucleoside reverse transcriptase inhibitor for the treatment of HIV; and Daklinza NS5A replication complex inhibitor, Sunvepra NS3 protease inhibitor, and Beclabuvir NS5B inhibitor. It sells products to wholesalers, retail pharmacies, hospitals, government entities, and medical profession. It has collaboration agreements with Nektar Therapeutics; Janssen Pharmaceuticals, Inc.; Biocartis Group NV.; and FameWave Ltd.
The company was formerly known as Bristol-Myers Company and changed its name to Bristol-Myers Squibb Company in 1989. Bristol-Myers Squibb Company was founded in 1887 and is headquartered in New York, New York.
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IndustryBiopharmaceuticals
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No. of Employees23, 300
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Website
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Jobs Posted1641
